The Technology
Behind OneBreath™
At Breath Diagnostics, our innovative OneBreath™ technology revolutionizes lung cancer screening. By capturing and chemically transforming volatile organic compounds (VOCs) in a patient’s breath, we deliver highly accurate, rapid, and cost-effective diagnostics—without radiation or invasive procedures.
OneBreath™ harnesses the power of your breath to unlock critical diagnostic information.
Unlike conventional screening methods that rely on imaging or invasive tests, our system uses a proprietary microreactor to chemically derivatize VOCs—the biomarkers linked to lung cancer—into detectable signals. This groundbreaking process offers not only speed and accuracy but also accessibility for a wider range of patients and clinical settings.
The OneBreath™ Process
Our test is simple. Just give one full breath into a bag.
Our device then concentrates and stabilizes your breath and the VOCs within it, onto our microreactor.
That chip gets sent to a lab, where it’s then analyzed by a LCMS system.
No radiation. No needles. No Waste.
Breath Collection
Patients provide a single, complete breath through our unique collection system in less than 30 seconds. This simple act forms the basis of our non-invasive testing procedure.
Microreactor Technology
Once collected, the breath is immediately transferred to our patented microreactor. Here, a specialized chemical formulation interacts with the exhaled VOCs, transforming these elusive compounds into stable, quantifiable markers. This derivatization process is the heart of our innovation—capturing even trace levels of biomarkers with unprecedented sensitivity.
Analysis & Processing
The chemically processed sample is then analyzed using ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS). With run times typically between 1 to 10 minutes, this state-of-the-art platform quantifies the biomarkers, enabling us to detect lung cancer at its earliest stages.
Results Delivery
Just like a blood test,, results can be provided to physicians and patients, in a timely and reliable manner that empower proactive intervention.
Proven in the Clinic
OneBreath™ isn’t just a promise—it’s been rigorously validated through clinical trials involving over 800 patients.
94% Sensitivity
Reliably detects early-stage lung cancer to enable prompt intervention.
85% Specificity
Minimizes false positives and reduces unnecessary follow-up procedures.
800+ Patients
Extensive clinical trials validate our technology’s impact across diverse populations.”
Multiple Global Patents
Protected innovations that secure our leadership in diagnostic advancements.”
Transforming Lung Cancer Screening
Experience a Paradigm Shift in Diagnostics with OneBreath™—where chemical innovation meets clinical precision to detect lung cancer early, safely, and cost-effectively.
Diagnosis with a Single Breath with Multiple Disease Potential
OneBreath quantitatively analyzes organic compounds to detect lung cancer using a single breath. But early research suggests it may be able to detect other diseases.
The Root of Breath Diagnostics with University of Louisville's Kevin Gardner
The University of Louisville has a long and proud tradition of getting new ideas out of the lab, field or studio and into the world in partnership with the private and public sectors or as new ventures.
The OneBreath Patient Process
In contrast to a Low-Dose CT Scan, OneBreath is a quicker and easier way of lung cancer diagnosis. Watch how simple our process is compared to the current standard of care, Low-Dose CT Scan.
Breath Diagnostics OneBreath Technology Overview
We have created a technology to quantify specific molecular markers in the Breath to diagnose disease -- starting with lung cancer.
Lung Cancer Diagnosis: Detecting Lung Cancer with a Single Breath
Lung cancer is difficult to diagnose because, for most people, there aren't any symptoms, especially at early stages. Breath Diagnostics is developing a new test detect lung cancer, using a non-invasive and simple procedure.
Innovating Beyond Conventional Diagnostics
Why OneBreath™ Stands Apart
Our technology is engineered to overcome the limitations of traditional lung cancer screening methods. With OneBreath™, you benefit from
High Accuracy
Clinical trials have demonstrated 94% sensitivity and 85% specificity—ensuring that early-stage lung cancer is detected reliably while minimizing false positives.
Non-Invasive & Patient-Friendly
A single, natural breath replaces the need for radiation-based imaging or invasive procedures, reducing patient discomfort and increasing screening compliance.
Cost-Effective & Accessible
Our streamlined process and portable design make advanced screening accessible to clinics, pharmacies, and community health centers, significantly lowering the cost per test compared to CT scans or PET imaging.
Rapid & Reproducible
The robust design of our microreactor and the efficiency of UHPLC-MS analysis ensure consistent, repeatable results—vital for clinical confidence and scalable diagnostics.
Advancing Diagnostics for a Healthier Tomorrow
Continuous Innovation: Ongoing R&D and strategic partnerships drive constant improvements in our technology, expanding its accuracy and diagnostic capabilities beyond lung cancer.
Scalable Impact: Our streamlined, cost-effective design enables widespread deployment in clinics and community health centers—paving the way for routine, accessible screening that transforms global healthcare outcomes.
Ready to Transform Lung Cancer Screening? Discover the Future Today.
Watch our video to learn how OneBreath™ is revolutionizing diagnostics with life-saving precision and simplicity.
